GrainGenes Assembly Report: D_Genome_Assembly_1.1
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| | Wheat D Genome Assembly 1.1 Feb. 2005. Fingerprints were obtained with SNaPshot Fingerprinting procedure. Fragments with sizes of 150-500 bp were used. On average, each CB unit represents ca. 1.9 kb in length. Five libraries, 277,091 Clones, 1691 Markers, 7447 Contigs |
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| | The contig merge was further performed based on the Assembly 1. Assembly 1 started at 1e-15, followed by step-wised DQing down to 1e-41. The clones resolved by DQing process were put back into singletons. The singleton(s) to contig end merging were performed at 1e-20. The contig end-to-end merging (requiring minimum 2 clones overlap at each end) at 1e-25 and at 1e-15 was performed at last. For Assembly_1.1 the contig end-to-end merging (requiring only one clone overlap at each end) was performed starting at 1e-25 and stepping down to the level of 1e-15. |
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